Jin Billy Li Associate Professor

Stanford University





研究方向:

Jin Li lab is in particular interested in RNA editing, a fine-tuning mechanism that introduces variations in RNA at the post-transcriptional level. One type of RNA editing common in metazoa, Adenosine-to-Inosine (A-to-I), is catalyzed by adenosine deaminases acting on RNA (ADAR). RNA editing is critical to life as the ADRA knockout mouse is embryonically lethal. Alteration in editing level of an individual substrate can also lead to serious pathological consequences as exemplified by the
glutamate receptor GluR2 (Brusa et al., Science 1995, 270:1677-1680). However, very few editing targets were identified in the nonrepetitive regions of the human transcriptome, which became one of the major barriers in the field. Technological
advance in DNA sequencing in the past several years has offered tremendous opportunities in the RNA editing field. It allowed us to discover hundreds of new genes with A-to-I editing sites (Li et al., Science 2009, 324:1210-1213). This has increased the human RNA "editome" by over a magnitude, but may still be tip of the iceberg. They will continue to comprehensively search for new editing substrates, by taking full advantage of new DNA sequencing technologies.The next big question is whether the RNA editing events are functional, and if so, what their specific roles are. They plan to perform a series of functional assays to screen many of the newly identified RNA editing genes, in both physiological and pathological contexts.

近期发表论文:

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Tan MH, Li Q, Shanmugam R, Piskol R, Kohler J, Young AN, Liu KI, Zhang R, Ramaswami G, Ariyoshi K, Gupte A, Keegan LP, George CX, Huang N, Pollina EA, Leeman D, Rustighi A, Goh YPS, The GTEx Consortium, Chawla A, Del Sal G, Peltz G, Brunet A, Conrad DF, Samuel CE, O‟Connell MA, Walkley CR, Nishikura K, Li JB (2017) Dynamic landscape and regulation of RNA editing in mammals. Nature, in press
Zhang R, Deng P, Jacobson D, Li JB (2017) Evolutionary Analysis Reveals Regulatory and Functional Landscape of Coding and Non-Coding RNA Editing. PLoS Genetics, 13(2): e1006563.
Ramaswami G, Deng P, Zhang R, Carbone MA, Mackay TFC, Li JB (2015) Genetic mapping uncovers cis-regulatory landscape of RNA editing. Nature Communications, 6:8194. doi: 10.1038/ncomms9194
Liddicoat B, Piskol R, Chalk A, Ramaswami G, Higuchi M, Hartner J, Li JB†, Seeburg PH†, Walkley C† (2015) RNA editing by ADAR1 prevents MDA5 sensing of endogenous dsRNA as non-self. Science, 349:1115-1120 [† equal contribution]
Ramaswami G, Zhang R, Piskol R, Keegan LP, Deng P, O'Connell MA, Li JB (2013) Identifying RNA editing sites using RNA sequencing data alone. Nature Methods, 10:128-132